TRα Polyclonal Antibody

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Catalogue Numbers: GCP12-50, GCP12-100

Sizes: 50µl, 100µl

Swiss-Prot: P10827

Host: Rabbit

Reactivity: Human,Pig

Applications: WB IF

Background: Thyroid hormone nuclear receptors (TRs) are ligand-dependent transcription factors which regulate and control many metabolic and developmental processes. There are two genes encoding TRs identified to date, TRα and TRβ. TRs bind to thyroid hormone response elements (TREs) with half-site binding motifs in the orientation of palindromes, direct repeats or inverted palindromes. The affinities of binding are both variable and influenced differentially by 3,5,3'-triiodo-L-thyronine (T3). Transcriptional regulation by TRs is also modulated by heterodimerization with TR nuclear accessory proteins, the most extensively characterized of which are the retinoid X receptors (RXRα, RXRβ and RXRγ). The TRα isoform TRα1 can display both a nuclear and undefined cytoplasmic location, and is the only TR that is imported into the mitochondrial matrix. TRα2 is a C-terminal variant of TRα1 that does not bind thyroid hormones (THs) and weakly binds DNA. TRα2 acts as a dominant negative antagonist of TH signalling.

Product: Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH7.2

Purification and Purity: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).

Specificity: TRα polyclonal antibody detects endogenous levels of TRα protein.

Note: For research use only, not for use in diagnostic procedure.

Extra Notes: Western blot (WB) analysis of TRα polyclonal antibody at 1:5000 dilution
Lane 1: THP-1 whole cell lysate (40ug)
Lane 2: K562 whole cell lysate (40ug)
Lane 3: The Kidney tissue lysate of Pig (40ug)
Lane 4: The Liver tissue lysate of Pig (40ug), Immunofluorescence analysis of HEK293T cells using TRα antibody at dilution of 1:50.

Bioworld Molecular Weight: ~ 58 kDa

Storage and Stability: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze-thaw cycles.

Alternative Name: Thyroid hormone receptor alpha; Nuclear receptor subfamily 1 group A member 1; V-erbA-related protein 7; EAR-7; c-erbA-1; c-erbA-alpha

Immunogen: Synthetic peptide, corresponding to Human TRα.

Conjugate: Unconjugated

Modification: Unmodified